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Image Search Results
Journal: bioRxiv
Article Title: High throughput intracellular delivery by viscoelastic mechanoporation
doi: 10.1101/2023.04.24.538131
Figure Lengend Snippet: (a) eGFP expression 24 hours after mRNA transfection to Jurkat cells for a range of extracellular mRNA concentrations. (b) T cell receptor (TCR) expression in Jurkat cells 2 days after transfection of Cas9 RNP complexes designed to knock out the TCR, for a range of RNP concentrations ( n = 3 for experimental conditions, n = 1 for controls). (c) Viability and delivery of 70 kDa FITC-dextran to HEK293T cells, 24 hours after processing, for three delivery buffer compositions and 2 different chip pressures (Unstretched, n = 2 ; PBS, n = 1 ; Cytomix buffers, n = 3 ). (d) Viability and delivery of 70 kDa FITC-dextran to primary activated T cells, about 90 minutes after processing, for increasing chip pressures ( n = 2 ).
Article Snippet: For mRNA transfection, we used an
Techniques: Expressing, Transfection, Knock-Out